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SRX6453222: Metagenome sequencing of perchlorate-reducing mixed culture B5
1 ILLUMINA (Illumina HiSeq 4000) run: 24.2M spots, 4.9G bases, 1.9Gb downloads

Design: Genomic DNA was extracted using a MoBio PowerSoil DNA Extraction Kit with a cell lysis protocol consisting of vortexing and heating at 70 C for 5 min, repeated twice (MoBio Laboratories, Inc., Carlsbad, CA). DNA library preparation and DNA sequencing were performed by the Adam Arkin Laboratory or the Vincent J. Coates Genomics Sequencing Laboratory at the California Institute of Quantitative Biosciences (QB3, Berkeley, CA)
Submitted by: University of California, Berkeley
Study: Genome-resolved metagenomics of marine perchlorate-reducing communities
show Abstracthide Abstract
Metabolic interactions and genetic exchange are important features of microbial communities. We performed genome-resolved metagenomics to characterize communities performing dissimilatory perchlorate reduction, a metabolism that involves reactive chlorine species and horizontal gene transfer of a conserved functional gene cluster. Distinct communities from source marine sediment and enrichments of varying salinity were recovered, and enriched populations included perchlorate-reducing bacteria as well as putative interacting populations of chlorate-reducing, sulfur-reducing, and fermentative microorganisms. Vectors for perchlorate reduction genes and a novel gene cluster involved in perchlorate reduction were also identified.
Sample: B5 mixed culture
SAMN11978086 • SRS5106564 • All experiments • All runs
Library:
Name: B5
Instrument: Illumina HiSeq 4000
Strategy: WGS
Source: METAGENOMIC
Selection: RANDOM
Layout: PAIRED
Runs: 1 run, 24.2M spots, 4.9G bases, 1.9Gb
Run# of Spots# of BasesSizePublished
SRR969489324,206,3844.9G1.9Gb2019-07-16

ID:
8549556

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